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Any further decrease in p K a will increase the thiolate concentration, but this effect will be undermined by the corresponding decrease in the nucleophilicity

Lactobacillus inhibits the growth of stray bacteria through rapid acid reduction and creates an anaerobic environment to reduce the oxidation reaction rate, so as to maintain the good structure and color of the silage

The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board

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Cloudiness, discoloration, or unusual odor indicates degradation