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When these engineered bacterial cells were incubated with 1 NF and exposed to light, they expressed MsrA and MsrB in comparable amounts, as shown by western blot analysis (Fig

Curr Chem Biol 3:2246

3.3 Short-chain fatty acids The structural integrity of the BBB is maintained by tightly joined brain endothelial cells, astrocytic end-feet, pericytes, and various transport proteins, which stringently regulate the penetration of substances from the blood into the brain tissue (103)

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After blocking in skim milk (5% w/v in phosphate-buffered saline with Tween 20 (PBST)), the membranes were incubated overnight at 4 C with specific primary antibodies