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Insulin receptor substrate (IRS)-2, not IRS-1, protects human neuroblastoma cells against apoptosis

These findings detail mechanisms of NRF2-dependent transcriptional regulation, pointing to noncanonical roles that extend beyond augmenting antioxidant capacity

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Start with shorter sessions (1015 minutes) and build up gradually, hydrating well before and after

The fact that Yap1 deficiency extends the egtA phenotype indicates that in wild-type, EGT limitation is partially compensated by other detoxification mechanisms that require activation by Yap1

once reconstituted, keep the solution at 28C and use within the standard research window, or aliquot and freeze at -20C to -80C for extended storage