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B12 supplementation has been reported to help with fatigue and nausea that semaglutide users commonly experience, though the evidence for this is largely anecdotal rather than clinical

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GoldBios Highest Density Nickel Beads are compatible with up to 20 mM of EDTA or DTT (Table 2), so if you cannot reduce these components in your buffer then that is probably the best form of nickel beads for your experiment