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To confirm equal protein loading and normalization, the membranes were probed with an anti-GAPDH antibody (1:10,000 dilution

The answer is yes
Sci Rep 7:9181

In addition to the lipotropic substances in these injections, they also include B12
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All cells were trypsinized and lysed, and the total proteins of each group were collected and determined using BCA (bicinchoninic acid) assays
