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25 Structuralactivity relationship (SAR) studies on 1-MQ showed that small substituents like methyl or amino groups on 1-MQ were tolerated, while bulky groups on 1-MQ lost binding affinity due to steric effects

Vitamin B12 can then be absorbed into the bloodstream via endocytosis in the distal ileum, or by passive diffusion in the absence of intrinsic factor.2 Pernicious anemia (an autoimmune disease) affects the gastric mucosa, leading to destruction of parietal cells and failure to produce intrinsic factor, resulting in vitamin B12 malabsorption and eventually deficiency

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NNMT is overexpressed in adipose tissue of obese subjects, and its inhibition has been explored as a strategy to modulate methyl group flux and NAD+ precursor availability