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Next, 20 g of protein was separated by 10% SDSPAGE (Sigma-Aldrich, 74255) using electrophoresis (200 V constant pressure) for 1 h and transferred to a nitrocellulose membrane (Merck Millipore, Billerica, MA, USA, IPVH00010)

ICF Immunodeficiencycentromeric instabilityfacial anomalies syndrome (ICF) is an extremely rare autosomal recessive disease that is characterized by profound immunodeficiency

doi: 10.4049/jimmunol.180.2.1088 220 KroetzDNDeepeGS

Bull Chem Soc Jpn 84:13621367

AKT kinases as therapeutic targets
