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10.3389/fphar.2020.01262 72 ShiL.HanQ.HongY.LiW.GongG.CuiJ.et al (2021)

Validation of RNA-Seq by Quantitative Real-Time PCR(qRT-PCR) Total RNA (2 g) from three biological replicates was purified and reverse-transcribed into cDNA using TranScript One-step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) with oligo(dT)

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Let the rich lather sit for a few moments to allow the active ingredients to penetrate before rinsing