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It is important to note that A negative Intrinsic Factor antibody result does not exclude the diagnosis of PA as only 60% of patients with PA will have this antibody

Al-Amin MM, Chowdury MI, Saifullah AR, Alam MN, Jain P, Hossain M, Alam MA, Kazi M, Ahmad A, Raish M, Alqahtani A

doi: 10.1016/j.neuron.2009.01.015

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Although ZO1 displacement is not sufficient to cause a barrier defect, the combination with other signalling pathways affecting tight junction structure, may contribute to a rearrangement of junctional complex, impacting on tight junction stability (114)