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Aberrant macrophage cytokine production is a conserved feature among autoimmune-prone mouse strains: elevated interleukin (IL)-12 and an imbalance in tumor necrosis factor-alpha and IL-10 define a unique cytokine profile in macrophages from young nonobese diabetic mice

2.19 Statistical analysis The data were analyzed using Microsoft Excel

Closing the pinhole too much decreases the specific signal and imaging requires higher laser power to excite the fluorophores which can eventually lead to photobleaching

They say ROS was eliminated via surface imperfections and unpaired electrons

The immunoblotted membrane was incubated with secondary antibodies conjugated with peroxidase for 60 min at RT