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Primary antibodies were removed and cells were washed with PBS

Many administrators prefer to use urine tests because of the accuracy and simplicity of the process

For athletes who have sustained injuries and are researching recovery options, the appropriate pathway is physiotherapy, approved pharmacological interventions (where relevant), and working with team medical staff on WADA-compliant protocols

VIP exerts its effects by binding to specific G-protein coupled receptors, notably VPAC1 and VPAC2 receptors, which are present in various tissues including the brain, gastrointestinal tract, and immune system [3]

Such mechanisms could elevate the risk of chronic inflammatory conditions, including cardiovascular diseases, malignancies, and other autoimmune disorders

However, it has some limitations