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Unlike cysteine, glutathione is not toxic, making it an effective option for a cellular thiol redox buffer, i.e
The mice received an intraperitoneal administration of isotonic saline (0.5 ml) to compensate for potential fluid loss
10.1038/s41569-019-0260-8 16
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First however, as with the measurement of GSSG above, it is necessary to first remove any GSH in the original sample with methyl methanethiosulfonate before reducing GSNO to release GSH
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