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-methylnorepinephrine is taken up into presynaptic vesicles, and released in place of norepinephrine

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Materials and methods Drugs and chemicals Powdered L-PAM and BSO (DL buthionine-( S,R )-sulfoximine) were purchased from Sigma-Aldrich (St Louis, MO, USA) and clinical grade BSO (L-buthionine ( S,R )-sulfoximine (50 mg/ml)) was provided by the National Cancer Institute (Bethesda, MD, USA)