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The protocols used for handling the mice were provided by the Qingdao University Center for Human Functional Experiments of the Medical College Animal Care Committee (Qingdao, China), which meets the guidelines set by the National Institutes of Health Guide for Care and Use of Laboratory Animals

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To assess oxidative DNA damage, we used two methods: immunostaining with antibodies to 8-oxo-dG, a marker of oxidative DNA damage [17], and the comet assay, a single cell-based technique that allows the researcher to detect and quantify DNA damage [22]

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