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It usually starts with weekly shots for a loading phase

Absorbance of reaction mixture was recorded at 734 nm by Spectrophotometer (Jenway 3300, UK) and percent inhibition was calculated by the following formula: Ferric reducing antioxidant power assay (FRAP) FRAP reagent was made by adding acetate buffer (300 mM, pH 3.6), TPTZ (10 Mm) in HCl (40 Mm), and ferric chloride (0.054 g, 20 mM)

Nat Rev Rheumatol 7(3):139150

United European Gastroenterol

Cycling different peptides offers one approach to maintaining sensitivity