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Microsomal lipid peroxidation: the role of NADPHcytochrome P450 reductase and cytochrome P450

Western blotting Cells cultured in 7H9, 7H9 with casamino acids or Sautons were spun down, resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol, and protease inhibitor cocktail (cOmplete, EDTA-free

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Rushing the escalation is the most common mistake researchers make, and it leads to severe nausea that could have been avoided

The tissue slices were rinsed in Tris-buffered saline and then incubated with the streptavidin-horseradish peroxidase reagent and a biotinylated secondary antibody for 30 min at 37 C

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