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Cell viability assay For the assay, 3.7 10 cells were seeded in 250 L of culture medium per well in 48 well plates and allowed to adhere for 24 h

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The SVCT2 has also been demonstrated in specialized and 1 ependymal cells termed tanycytes that line the ventricular walls in the area of the hypothalamus [57]

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To amplify cDNA fragments, primer pairs (Table 2) were used for RT-PCR

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