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0.5 TrypLE Select solution was prepared with 50% TrypLE Select, no phenol red (Thermo Fisher Scientific) and 0.25 mM EDTA (0.5 mol/L EDTA Solution (pH 8.0), Nacalai Tesque Inc.) in DPBS () for single-cell culture

Plasmid construction, growth and purification The pCTV plasmid containing the gene sequence encoding S protein, used in the immunization of experimental animals, was developed at the Centro de Tecnologias de Vacinas (UFMG)

doi: 10.2337/diacare.22.12.1971 178 DruweILSollomeJJSanchez-SoriaPHardwickRNCamenischTDVaillancourtRR

doi: 10.1016/0304-3940(83)90445-7 68 GagnonJAniniY

Der Ehrlich-Aszites-Tumor (EAT) ist ein Tumor, der in allen Stmmen von Musen wachsen kann