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class IV FINs induce ferroptosis by exacerbating iron overload or activating HO-1 (Hassannia et al., 2019)
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CellTiter-Glo reagent (3 mL) was diluted in 9 mL of DPBS, and 100 L of this solution was added to each well on top of culture media

After acidic hydrolysis (2 S ,3 S ,4 R )-3,4-dihydroxyglutamic acid [(2 S ,3 S ,4 R )- 4 ] was obtained as the hydrochloride
The activity of GPX was determined by catalyzing 1 nmol GSH per minute per mg in samples
