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some clinical evidence and small-scale clinical studies have reported improvements in motor symptoms, sleep disturbances, and quality of life with cannabinoid-based therapies [56]

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The Parkinsons-associated protein DJ-1 regulates the 20S proteasome

To determine the effects of stimulating TCA cycle activity, we treated hepatocytes with dichloroacetate, which stimulates substrate entry into the TCA cycle by inhibiting PDKs (Constantin-Teodosiu, Simpson, & Greenhaff, 1999

Afterwards, the cells were blocked, permeabilized and incubated with primary mouse monoclonal antibody to H2AX (Ser-139) at 1 : 1000 (Upstate Biotechnology, Upstate, NY, USA) and diluted 1 : 500 for 1 h at room temperature, followed by incubation with anti-mouse FITC secondary antibody (Sigma-Aldrich) that was diluted 1 : 200 for 1 h at room temperature
