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The results showed that the proportion of SA--gal positive cells after OGD treatment was significantly higher than that in the control group (Figure 3D)
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Insert needle at a 45 angle (or 90 if using very short needle and ample subcutaneous tissue) into the subcutaneous fat layer [11] [12]
RNA 10, 1218
Human umbilical vein endothelial cells (HUVEC, Lonza) were cultured in endothelial cell basal medium (EBM, Lonza) supplemented with endothelial cell growth medium (EGMSingleQuots, Lonza), 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin and 100 g/mL streptomycin
Next, using a polyurethane cannula (2 mm in diameter), 2 ml of AA (3%) in normal saline (0.9%) was intrarectally delivered for 30 s
