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Briefly, cells were kept in growth medium consisting of DMEM with 10% FBS, 10 mM HEPES, 2 mM L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin (all from Thermo Fisher Scientific Waltham, MA, United States), and 2.5 ng/ml hFGF-2 (all Sigma St