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RFP fluorescence (excitation= 584 nm, emission= 607 nm) and mCherry fluorescence (excitation= 587 nm, emission 610 nm) was measured using a Thermo Fisher Varioskan LUX multimode microplate reader

This region encompasses a clearly visible phenylalanine (F917) in the cryo-EM structure, but a movement of the downstream region of the -helix, possibly due to a different overall conformation of the entire structure

Verdery, R.B., and Goldberg, A.P

- Those on anti-psychotic medications (e.g., Haloperidol) or chemotherapeutic drugs (e.g., Cisplatin)

Probing treatment response of glutaminolytic prostate cancer cells to natural drugs with hyperpolarized [5-(13) C]glutamine

Now consider what she replaced it with