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Immunoblotting and immunoprecipitation assays The cultured cells were rinsed once with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 mM HEPES (pH 7.0), 1% Triton X-100, 150 mM NaCl, 10% glycerol, 1 mM EDTA, 2 mM EGTA, 1 mM DTT, 5 mM Na 3 VO 4 , 5 mM NaF, 1 mM AEBSF, aprotinin (5 g/ml), and leupeptin (5 g/ml)

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84 However, another study performed in B cells of lupus-prone mice showed that glutaminolysis requirement is not B cell-intrinsic

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Free Radic Biol Med 1995, 19 (2), 227250

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Figure 1 3 Conclusion and future perspectives CKD is a debilitating illness that increases the risk of cardiovascular problems and is typified by persistent inflammation

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