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Long-term effects of KD The long-term safety profile of KD remains a concern due to its unconventional macronutrient distribution, characterized by high fat and low carbohydrate intake

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Furthermore, its ability to modulate the PTEN/AKT pathway and enhance the efficacy of chemotherapy drugs such as sorafenib underscores its potential in combination therapies

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Thin sections were incubated overnight, washed in PBS, and incubated with biotinylated rabbit anti-mouse secondary antibody (Dako, UK) at 1:200 dilutions for 1 h