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Propofol is used to initiate and maintain general anesthesia and sedation in patients undergoing surgery

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Cell pellets were resuspended in 500 l of IP buffer 1 [50 mM HEPES pH 7.6, 125 mM KCl, 0.1% NP-40, 20% glycerol, 1 mM EDTA, 1 mM PMSF, and Complete protease inhibitor cocktail (Sigma)] and disrupted using Mini-Beadbeater-16 (BioSpec Products)

Abstract Endoplasmic reticulum (ER) stress is associated with dysregulated metabolism, but little is known about how the ER responds to metabolic activity

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Characteristics of the Study Population The characteristics of the study population also strongly influenced outcomes