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This series of changes is mainly driven by a significant reduction in the expression of the key transcriptional coactivator, peroxisome proliferator-activated receptor coactivator 1 (PGC-1)

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By blocking NNMT activity, the compound preserves nicotinamide availability for the NAD+ salvage pathway, leading to 1.2- to 1.6-fold increases in intracellular NAD+ concentrations