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Recombinant production of medium- to large-sized peptides in Escherichia coli using a cleavable self-aggregating tag
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coli BL21(DE3) using 0.5 mM IPTG induction at 37C for 6 h and purified initially using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva)
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[DOI] [PubMed] [Google Scholar] [46].Saita S, Ishihara T, Maeda M, Iemura S, Natsume T, Mihara K, et al
