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After a wash step, cells were incubated with fixation/permeabilization concentrate for 30 minutes to fix the surface and permeabilize cells for intracellular staining
doi: 10.1158/1940-6207.CAPR-15-0372 75 WangS.ZhangG.ZhangP.MaX.LiF.ZhangH.et al

Cellular subpopulations, including neutrophils, monocytes, and lymphocytes, were defined based on their forward and side scatter properties and gating strategies were validated

doi: 10.1007/s11240-025-03253-6 Summary Keywords artificial intelligence, assisted reproductive techniques (ART), environmental pollutants, male infertility, semen quality Citation Jagadesh P and Sridharan TB (2026) Environmental determinants of male infertility: emerging threats and technological interventions

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