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Nat Struct Biol 1(1):3647

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Double-stranded DNA was obtained by annealing equimolar concentrations in an annealing buffer (25 mM Tris and 100 mM NaCl [pH 8.0])

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Thus, nLDs in young nuclei likely form at the envelope, displacing peripheral heterochromatin as they grow into the interior, but many nLDs in older nuclei appear to form in the interior

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