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Next, the slides were applied with the PLUS and MINUS PLA probe solution for 1 h at 37 C and performed with ligation and amplification by using Duolink In Situ Detection Reagents Red

Before the targeted synthesis and isolation of these peptides, research into tissue recovery and regenerative processes relied heavily on generalized growth factors and less specific biological agents

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Blood samples were collected at specific time points during the study to analyze immune parameters