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In previously published protocols different types of derivatization reagents (CMQT or CMLT), different pH of the buffer used in the protocols (7.4) and shorter time of sample reduction and derivatization (3 min) were applied (Tables 3 and 4.) Changing the conditions of reduction and derivatization increases the efficiency of this process, which may give higher final concentrations of the analytes

The elevated dyslipidemia recorded in the AZA group, evidenced by a considerable rise in serum triglycerides and total cholesterol, a significant drop in HDL, and a significant increase in LDL, was a precursor to fatty liver and lipotoxicity, as previously suggested (50)

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Developmental Biology, 226, 118136

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