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If our data based on an intense and short exposure to HNE can be extended to an in vivo situation where low concentrations of HNE are produced on a long time scale, the present results suggest that although the amount of DNA adducts is low upon treatment by exogenous HNE, their formation could be favored upon oxidative stress

Left gel shows very little GST-fusion protein in the flow-through and a lot in the elution indicating that sufficient glutathione agarose resin was used in this purification