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Mice treated with GHK were able to find the escape hole significantly faster in trials 4 and 5 compared to mice treated with saline (Figure 2) as assessed by a Box Maze spatial navigation learning task [27] at the end of the treatment period

therefore, tissues should be minced into small pieces and rinsed thoroughly in ice-cold PBS (0.01M, pH 7.4) to remove excess blood

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As such, high intakes of methylxanthines may also contribute to improved weight loss and weight maintenance through enhanced fat oxidation and thermogenesis (137)