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Prepare the HisLink Binding, Wash and Elution Buffers Note: Polyhistidine-tagged proteins can be eluted using 2501,000mM imidazole

32 The authors used selfadministered questionnaires including neuropathic pain scores, visual analog scale (to quantify pain), SF12 (to quantify overall health), UCLA activity scale (to quantify activity level), and the WOMAC (to quantify pain, stiffness, and physical function) as their outcome measures

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