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HCT116 cells stably expressing H2B-mCherry were obtained by transfection of these cells with the pcDNA3-H2B-mCherry (Addgene plasmid 20972) 35 expression plasmid using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and antibiotic selection of G418-resistant clones (1 mg/ml)

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The RASRAFMEK pathway sensitizes cancer cell lines with RAS to ferroptosis via mitochondrial voltage-dependent anion channels 2/3 (VDAC2/3) (Yagoda et al., 2007)