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The CNBr method utilizes relatively mild conditions for ligand attachment, making it suitable for many sensitive biomolecules

Cell proliferation assay The viability of GC-1 spg, TM3 Leydig, and TM4 Sertoli cells was measured using an MTT assay EZ-Cytox kit (Daeil Lab Services Co., Seoul, Korea) according to the manufacturers instructions Each cell was seeded in 24-well plates at a density of 0.5 10 5 cells/well and incubated at 37 C for 12 h

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