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Exogenous AmgK and MurU expression plasmid pBBR-KU was constructed from pBBR1MCS vector, using the PCR product of amgK and murU cluster, and the 5-KpnI and 3-HindIII restriction sites (primer set: pBBRKU-For/pBBRKU-Rev)

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The name and address of the APIs manufacturer can be found on Letcos Certificates of Analysis, and the manufacturers FDA registration status can be verified on the FDAs Drug Establishment register

the CAR T cells described herein can undergo robust in vivo T cell expansion and can persist for an extended amount of time

4l, Supplementary Data 5a), we confirmed that rapamycin inhibited mTOR-MYC signaling/targets as well as downregulated all major metabolic pathways (OXPHOS, glycolysis and fatty acid metabolism) and T cell activation/effector pathways (IL-2-STAT5, IL-6-JAK-STAT3, allogenic activity, and interferon response)

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