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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)

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Endocrinology (2006) 147(2):100713

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None of this is difficult once you have done it twice, and the calm that comes from a clean routine makes the whole experience feel ordinary rather than clinical