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To further expand this knowledge, we examined the effect of SeMSC or selenite (ranging from 02 to 5 m) either individually or in combination with the ITC SFN or iberin (ranging from 6 to 8 m) for up to 12 d on factors modulating gene-specific ( p16 INK4A , ESR1 ), global (long interspersed nuclear element-1) methylation (a surrogate marker of genome-wide methylation), and DNMT expression, in two colorectal cancer cell lines namely Caco-2 and HCT116