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GABAB and zinc are extremely negatively correlated with stereotyped and repetitive behaviors ( 4.2 Abnormal GABA levels in the brain tissue of ASD patients Postmortem examination, single-photon emission computed tomography (SPECT), and magnetic resonance spectroscopy (MRS) have been employed to conduct in-depth studies on the brains of ASD patients

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A single colony was used to inoculate 200 ml LB broth containing 50 g/mL kanamycin and incubated at 37C until OD 600 reached to approximately 0.5