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This imbalance of the gut microbiota and continued exposure of the colonic epithelial cells to urea is harmful to intestinal epithelial barrier function as it aids the disruption of the mucus layer and lowers the expression of tight junction proteins (Vaziri et al., 2013

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We then measured the abundance of ECHS1 mRNA in cells cultured under normoxia or hypoxia by quantitative real-time PCR and showed that ECHS1 transcription was upregulated by hypoxia (Supplementary Fig

Yadav H, Sharma U, Singh S, Gupta Y (2019) Effect of Tribulus terrestris in mercuric chloride-induced renal accumulation of mercury and nephrotoxicity in rat

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This PDMS layer was treated with oxygen plasma (Harrick Plasma, Ithaca, NY, United States) for 90 s to improve the bonding of the PDMS layer onto the glass substrate and also to make the device hydrophilic for easy cell and culture medium loading