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Cytotoxic activity of TMZ and QN, alone or in association, in neutral and acidic pH Since it has been reported that CQ was unable to block the autophagic flux at acidic pH 31 , and considering that acidic pH is an important feature of tumor microenvironment and a major determinant of tumor progression, we evaluated the cytotoxic effects of QN, TMZ, and their association in GSC#1 cultured at pH 6.5

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