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10.1016/0378-8741(93)90005-P 11 AnM

Lysed bone marrow cells were stained with biotin-conjugated lineage marker antibody cocktail:CD3 monoclonal antibody (ThermoFisher Scientific, #13-0037-82, 1:200 dilution), CD4 monoclonal antibody (ThermoFisher Scientific, #13-0041-85, 1:100 dilution),CD11b monoclonal antibody (ThermoFisher Scientific, #13-0112-85, 1:100 dilution), CD5 monoclonal antibody (ThermoFisher Scientific, #13-0051-85, 1:200 dilution), CD8 monoclonal antibody (ThermoFisher Scientific, #13-0081-85, 1:200 dilution), CD45R (B220) monoclonal antibody (ThermoFisher Scientific, #13-0452-85, 1:200 dilution), Ly-6G monoclonal (ThermoFisher Scientific, #13-5931-85, 1:200 dilution),TER-119 monoclonal antibody (ThermoFisher Scientific, #13-5921-82, #1:200 dilution), at 4 C for 30 min

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IRBPs: either a translational enhancer or a translational inhibitor, binding to iron Iron A metallic element with atomic symbol fe, atomic number 26, and atomic weight 55

To reaffirm our findings, we also performed SEC with hPRDX6 at both lysosomal and cytosolic pH

This observation means that xCT blockage induces the accumulation of glutamate that is converted into -ketoglutarate instead of being used in glutamate-cysteine exchange and consequently that is why cysteine does not enter the cell and give rise to glutathione