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1,25-Dihydroxyvitamin D3 reduces TGF-3-induced fibrosis-related gene expression in human uterine leiomyoma cells

Real-time monitoring of intracellular radical load In total, 25,000 APEX2-expressing HeLa cells, suspended in FluoroBrite (Gibco, A1896701) with 2% FCS, were seeded per well into opaque white 96-well plates (Thermo Fisher Scientific, 136101)

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Inhibition of this pathway under normal conditions phenocopied a hyperglycemic state by enhancing chemotherapeutic efficacy in mouse PDAC, while rescuing the pathway under high glucose abrogated the anti-tumor effects observed with chemotherapy