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After a wash step, cells were incubated with fixation/permeabilization concentrate for 30 minutes to fix the surface and permeabilize cells for intracellular staining
O., Pickholz, M., Remko, M., Saviano, M., Skwarczynski, M., Tang, Y., Wolf, W

With 5 mL of water in a small vial, you may only get 40-50 draws before the vial is empty

Vascular endothelial growth factor (VEGF)-key factor in normal and pathological angiogenesis