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[DOI] [PubMed] [Google Scholar] 84.Fox J.H., Kama J.A., Lieberman G., Chopra R., Dorsey K., Chopra V., Volitakis I., Cherny R.A., Bush A.I., Hersch S

According to the current guidelines, the folic acid derivative, leucovorin together with 5-FU combined with oxaliplatin (FOLFOX) or 5-FU combined with irinotecan (FOLFIRI) are recommended as the first-line therapy for patients with mismatch repair proficient (pMMR), microsatellite stable (MSS) metastatic CRC (mCRC) (Morris et al., 2023)

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In the second step adding NADPH and glutathione reductase results in conversion of all GSSG to GSH which reacts subsequently with DTNB and fluorescent or colorimetric substrate to be quantified