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The gut microbiota is a complex ecosystem that has a major impact on human health via interactions between microbial composition and metabolic activity

For example, in human experiments conducted by Tang et al., TMAO plasma levels were markedly decreased after administration of antibiotics but returned to the previous level after withdrawal of antibiotics (59)

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Metabolite treatment in cells 293T cells were plated in 6-cm dishes and cultured in Dulbecco Modified Eagles Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (PS)

L-type amino-acid transporter 1 as a novel biomarker for high-grade malignancy in prostate cancer
Thiamin Fact Sheet for Health Professionals. Available at: MedlinePlus