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J Ethnopharmacol 282:114594 Nishi Y et al (2001) Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor

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Additional studies that incorporate parameters such as buffer type, surfactants, salt concentration, pH and other excipients should be evaluated for their impact on the proposed detection mechanism

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For analysing DNA/RNA content, cells were collected and stained for surface markers before being fixed with 4% paraformaldehyde for 30 min at 4 C, followed by a permeabilization step with FoxP3 permeabilization solution (eBioscience)