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The influence of substituents on acidity is more pronounced in DMSO than in water, so Hammett parameters derived from aqueous p K a values actually underestimate substituent effects in reactions performed in non-hydrogen bonding media

The precleared lysate was then incubated with z13-conjugated agarose beads, which were prepared by conjugating cys-z13 peptide (1 mg) with sulpho-link agarose beads (1 ml, Pierce)

Therefore, it serves as a valuable tool in advanced research environments requiring precision and consistency

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